Confocal microscope - Zeiss LSM 780 NLO
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Cheney D - 1st floor
The Zeis LSM 780 NLO is a confocal microscope with high-sensitivity spectral light detection. It is set-up on inverted AxioObserver microscope dedicated to live cell imaging, thanks to a dark incubation chamber and modules controling CO2 and temperature. The pulsed multiphoton laser (coming in april 2012) will provide imaging into thick samples.
CRCL's LSM 780 NLO confocal microscope
- Equipment -
- LSM 780 confocal scanner with a 34 channels spectral GaAsP detector mounted on an inverted AxioObserver. Transmitted light detector.
- XYZ motorized stage, with fast Z-piezo.
- Objectives: Plan-Apochromat 10X, 20x, 40x water immersion, and 40x, 63x, 100x oil immersion.
- Coherent Chameleon Vision S pulsed multiphoton laser
- Incubation chamber with control of temperature and CO2
- Perfusion (coming soon) - A CO2 incubator is available.
- Off line analysis P, for post-acquisition processing, including object tracking.
- Lasers -
Diode laser 405nm
Laser Argon 458nm / 488nm / 514nm (25mW)
Diode laser 561nm
Laser He/Ne 633nm
"biphoton" pulsed laser - Coherent Chameleon Vision S
- Applications -
- Conventional confocal imaging (multi-colors, 3D, colocalization)
- Spectral imaging, in live and post-acquisition mode
(autofluorescence, probes with close emission spectra, single pass multi-color imaging).
- Imaging of dynamic events, "F" methods (FRAP, FLIP, FRET), photo-activation.
- Time Lapse, compatible with multipoint reading, tiling Z-stack.