Accueil > Technological platforms > Flow cytometry core facility
Flow cytometry core facility
The flow cytometry platform of the CRCL is part of the SFR Lyon-East platforms (CYCLE). It offers help and advice to the scientific community on state-of-the-art equipment including flow cytometry, cutting edge full spectrum cytometry and cell sorting.
Since 2020, the platform is dedicated to ensuring a high quality approach to meet the needs of stringent requirements in data output.
The platforms provides support and expertise to researchers aiming at developing projects in flow cytometry, from panel conception to data interpretation.
The cytometry platform works closely with the PI3 immuno-monitoring platform for the development of translational analysis. We share expertise in setting up panels with high information content and analyzing datasets in order to optimize the development of cross-sectional studies conducted by the P3i platform.
Contacts
Localization : 3rd floor of the Cheney B building – Access details
Mailing adress : cytometrie@lyon.unicancer.fr
Telephone : 04 78 78 29 12
Members of the steering committee of the platform
POURCHET Julie, BIOTA Cathy, BERTOLINO Philippe, CARAMEL Julie, KANIEWSKI Bastien, VALLADEAU Jenny, COSSET Erika, GABUT Mathieu, RUBY Samia, GRINBERG-BLEYER Yenkel, BERTHENET Kévin, HENNINO Anca, JACQUEMETTON Julien,
CASCALES Elodie, PLISSONNIER Marie-Laure, LEFORT Sylvain, SOUDJA Saidi, GOLDSCHNEIDER David,
PETRILLI Virginie, DURET Cédric, VANBERVLIET Béatrice, BARTOSCH Birke
Equipments
The cytometry core facility has a wide range of cytometers and multicolor sorters
dedicated to research and longitudinal analysis.
Détail des configurations
Access the booking website
Sorting request form
Platform operation
Fees for academic researchers
Fees for industrial or private companies
Introduction to Spectroflo
Introduction to DiVa
Introduction to FlowJo
2023 Key data
Actives users
Hours of analyses
Hours of spectal analyses
Hours of charged sorting
Trained people
Resources
- Pack Design Panel Aurora
Fluorophore-equivalence-aurora
liste sondes par laser
Panel-design-matrix Aurora - Optimized Multicolor Immunofluorescence Panel
OMIPs – human panels
OMIPs – mice/rats panels
OMIPs – monkey panels
- Spectra viewvers
Available fluorochromes can vary depending on the site. If the fluorochrome of interest is not available spectraviewer, try another!
Fluorescence SpectraViewer | Thermo Fisher Scientific – FR
Fluorescent Spectraviewer | Bio-Rad (bio-rad-antibodies.com)
Cytek Full Spectrum Viewer (cytekbio.com)
Spectrum Viewer | BD Biosciences-US - Probe performance
Comparison of SI between Aurora and Fortessa