Research Pathology Platform East

The Research Pathology Platform East provides researchers from the CRCL and the CLB, as well as private companies, with standard and innovative histopathological techniques for in situ molecular detection of proteins and mRNAs in human or animal samples. It promotes collaborations between researchers, clinicians and pathologists from the CLB, the HCL CHU Lyon-Est and VetAgroSup.

How to contact us ?

Location : Cheney B building, 3rd floor, Léon Bérard Center

E-mail : anapath.recherche@lyon.unicancer.fr

Platform organization :

– Sylvie Lantuejoul, scientific coordinator
– Nicolas Gadot, operational and technical manager
– Francesca Damiola, chief of project for Nanostring DSP technology
– Julie Valantin, expert in immunohistochemical (IHC) techniques and in situ hybridization (ISH)
– Laura Francols, expert in standard anatomical pathology techniques and Quality standards
– Clémentine Le Nevé, expert in multiplex immunofluorescence (IF) and NanoString (DSP)
– Elodie Voilin, image analysis and DSP referent

Technologies available

Anatomical pathology technique to characterize the morphology of tumor tissues or cells derived from humans or from animal models, either frozen or fixed, using standard or custom staining (automated platform for dehydration LeicaASP6025, paraffin embedding, microtomographyand staining LeicaST5010-CV5030)
Automated immunohistochemical/immunocytochemical techniques, chromogenicor fluorescent staining, In Situ Hybridization(ISH): Automated Roche Discovery XT, Roche Benchmark ULTRA, Leica Bond RX
Multiplex immunohistochemical technique following the acquisition of the VectraPolaris Akoya platform in 2019
Digital pathology platform including a brightfield and fluorescent 3D HISTECH slidescanner
Image analysis platforms (n=3) equipped with the Halo Indicalabs and Inform software
Production of TMA (Tissue MicroArray) using the Tissue microarrayers (ExciloneMinicore)
Macro/microdissection using the PALM Laser UV (Laser MicrodissectorLeicaLMD6)
Digital Spatial Profiling (DSP) Nanostring technology acquired in 2020

Innovative technologies

The Vectra Polaris Akoya Platform :

This platform can be used to perform 6-8 multiplex fluorescence immunolabeling on a single FFPE (Formalin-FixedParaffin-Embedded) tissue section, based on a sequential IHC methodology that uses tyramide-fluorophore signals.

This methodology relies on a strong expertise IHC techniques and prevents issues linked with cross species reactions . The Vectra Polaris scanner acquires images on up to 9 fluorescent channels. Spectrum quantification and marker analysis can then be conducted using the Inform or Halo software.

The GeoMx Digital Spatial Profiling (DSP) NanoString Platform :

This innovative technology provides a spatial quantification of over over 96 proteins or up to 18000 RNAs on a single FFPE tissue section of pre-identified regions of interest ranging from several cells to 600 μm in diameter.
This technique combines : 1- morphological and IHC approaches to determine the regions of interest, 2-an IHC-or ISH- like technology for the hybridization of antibodies or of oligonucleotide-tagged probes, 3-a quantification step of these oligonucleotides to obtain aspecific read-out of the abundance of each protein or RNA.

Access to the platform

If necessary the platform can provide technical assistance for a particular step of your project, or can manage your project entirely.

Our collaboration with researchers is a key aspect of our work and should be instigated at the onset and followed through out the project. For studies using patient cohorts, the involvement of a pathologist is crucial, for patient selection, marker validation, and project follow-up. Of note, it is very important to comply with the rules and regulations of the RGPD (General Rules and Regulations for Data Protection).

From a practical point of view, you should contact the platform via e-mail anapath.recherche@lyon.unicancer.fr at the beginning of your project and send them a document summarizing your project. The microdissection and image analysis platforms are open to users following a training period and by complying with the booking system. All of the information you may need to submit a project, as well as the protocols used are available at V:\CRCLPlateformes\FormulairesAnapathRecherche (CLB network only).

Advice for sample preparation

Delay for fixation: as soon as possible following the sacrifice
Fixatives : 10% neutral buffered formalin, PFA: 4%
Fixation volume: should be mini-mum 10X the size of the sample
Vials : wide mouth container, NO 15ml tubes!
Fixation duration : 24 to 48h
Identification : readable, clear and detailed

Processed samples: animal samples, human specimens, cells, organoids, 3D structures

Mention us in your publications!

To allow our platform to pursue its objectives, we need you to mention our work in your publications as follows:

Plateforme Anatomopathologie Recherche, Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), Lyon, 69373, France

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