Approaches & applications
At the DNA level, search for genetic alterations of SNV (substitution, insertion, deletion) or CNV (genomic copy number):
- Whole genome sequencing (WGS): Sequencing of the whole genome
- Whole exome sequencing (WES): Sequencing of all coding sequences
- Panel of genes of interest: a group of genes dedicated to a specific study
- Sequencing of small targeted fragments: Sanger method
At the DNA level, study of the interaction between proteins and DNA
- Chip-seq
At the RNA level, from a population of cells (bulk-ARN ) or sorted cells (microbulk), explore the expression of all the transcripts (transcriptome), and/or the search for fusion transcipts:
- RNAsequencing: total RNA method, polyA capture or exome capture
From a specific RNA population, analyze ribosome methylation status:
- RiboMethseq
From a tissue slide, study the expression of a panel of genes:
- System EdgeSeq (HTG): targeted commercial panels (RNA or miRNA)
At the level of single cells, new technological developments allow the analysis of gene expression and thus, for example, to characterize more precisely cell heterogeneity or the mechanisms linked to the development of tumor cells. Through the implementation of multiomics analyses, it is currently possible to add biological information, such as the expression of surface proteins or the expression of B/T cell receptors (BCR/TCR)
- scRNAseq
- CITEseq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing)
- BCR / TCR seq
